FastQCFastQC Report
Wed 31 May 2023
EGAF00007914753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296969
Sequences flagged as poor quality0
Sequence length27-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC6570.22123521310305114No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT5710.1922759614639912No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA5620.18924534210641517No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5170.17409224531853493No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5140.17308203886600956No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG4790.16129629691988054No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA4610.15523505820472844No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG4560.15355138078385286No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4350.14647993561617542No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT4300.14479625819529984No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT4160.14008196141684823No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4100.13806154851179753No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4020.13536766463839658No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG3950.1330105162491708No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG3770.12694927753401872No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3720.12526560011314314No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3650.1229084517239173No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3610.12156150978721685No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3580.1205513033346915No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC3540.11920436139799104No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA3490.11752068397711546No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3460.11651047752459011No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT3460.11651047752459011No Hit
CTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGACATGC3380.1138165936511892No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3330.11213291623031361No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3220.10842882590438732No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3210.1080920904202122No Hit
CTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGTGGAG3210.1080920904202122No Hit
TGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCATG3180.10708188396768686No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT3180.10708188396768686No Hit
CATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTA3170.10674514848351174No Hit
ATGTGGAGATGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3160.10640841299933664No Hit
CCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTC3110.10472473557846106No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3080.1037145291259357No Hit
CCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCCACA3070.10337779364176058No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3010.1013573807367099No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3010.1013573807367099No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC2990.10068390976835966No Hit
CATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCT2990.10068390976835966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)