FastQCFastQC Report
Sun 21 May 2023
EGAF00007914754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286731
Sequences flagged as poor quality0
Sequence length19-142
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7100.247618848328224No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6200.2162305436105618No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA4660.16252166664922874No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT4530.1579878004122331No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4240.1478737911143197No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT4130.14403744275993877No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4100.14299116593601668No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC3960.13810854075771367No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC3930.13706226393379162No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC3870.13496971028594745No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG3840.13392343346202537No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG3720.1297383261663371No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3690.12869204934241502No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA3660.12764577251849293No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG3550.123809424164112No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3460.12067059369234578No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3390.11822928110319429No Hit
GTACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGG3310.11543920957273542No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC3310.11543920957273542No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA3240.11299789698358392No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3240.11299789698358392No Hit
CTTCACAGCCCTGCGTAAACGTCCCTGTGCTAGGTCTTTTGCAGGCACAG3190.11125410227704714No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT3150.10985906651181769No Hit
CCTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACC3150.10985906651181769No Hit
GTACTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTC3060.10672023604005147No Hit
CTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCG3060.10672023604005147No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3000.10462768239220732No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG2990.10427892345089998No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC2990.10427892345089998No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT2990.10427892345089998No Hit
TACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGGG2960.1032326466269779No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT2950.10288388768567053No Hit
CTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACAGGCCT2930.10218636980305583No Hit
GTCTTTTGCAGGCACAGCTTTTCCTCCATGAGTACGTATTTTGAAACTCA2900.10114009297913375No Hit
CTTTCCAGCATGGTGAGGGCTGAGGTGACCCTTGTCTCTGTGTTCTTGTC2880.10044257509651904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)