FastQCFastQC Report
Sun 21 May 2023
EGAF00007914755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285462
Sequences flagged as poor quality0
Sequence length20-142
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC6880.24101281431504018No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA5740.20107755147795503No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT5260.1842627039676034No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5020.17585528021242758No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG4620.16184290728713455No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG4620.16184290728713455No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4540.15904043270207593No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA4530.15869012337894361No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4500.15763919540954663No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT4490.1572888860864143No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4300.15063300894690013No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4100.1436268224842536No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA4090.14327651316112128No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC4040.14152496654545962No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT4010.14047403857606267No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3970.13907280128353336No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3620.12681197497390195No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC3540.12400950038884336No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3490.12225795377318172No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3440.12050640715752009No Hit
CCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTC3400.1191051698649908No Hit
TATAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTG3380.11840455121872615No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3300.11560207663366753No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCAC3260.11420083934113821No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3210.1124492927254766No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3210.1124492927254766No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3180.11139836475607963No Hit
CTTTGCGATCTGCACACACCAGTTGAGCAGGTACTGGGAGCCAATATTGT3120.10929650881728566No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA3090.10824558084788868No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT3090.10824558084788868No Hit
CCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACAT3070.10754496220162404No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3060.10719465287849171No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3020.1057934155859624No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3010.10544310626283007No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT3010.10544310626283007No Hit
CTTCTCCACTGGGTGTAAGAGGCTCCACAAGCTGGGGGGGACAAGAACAC2970.10404186897030078No Hit
AAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAAAGCCA2940.10299094100090381No Hit
CCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACC2900.10158970370837449No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC2870.10053877573897751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)