FastQCFastQC Report
Wed 8 May 2024
EGAF00007914766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424873
Total Bases58.5 Mbp
Sequences flagged as poor quality0
Sequence length21-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA6410.15086861250303032No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT5680.13368700764699099No Hit
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC5560.13086263424599823No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5420.12756753194483997No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC5390.12686143859459179No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC4750.11179811378929704No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC4740.1115627493392143No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG4680.11015056263871792No Hit
CCCTGAATTCGGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAG4680.11015056263871792No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4610.10850301148813882No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA4610.10850301148813882No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA4450.10473718028681511No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG4310.1014420779856569No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4290.10097134908549142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)