FastQCFastQC Report
Wed 31 May 2023
EGAF00007914768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322970
Sequences flagged as poor quality0
Sequence length22-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7090.21952503328482523No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6970.21580951791188036No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5480.1696752020311484No Hit
GTCCACGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC5050.15636127194476268No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4980.1541938879772115No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA4690.14521472582592812No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4580.14180883673406197No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC4550.14087995789082577No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG4200.13004303805306994No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4160.128804532928755No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG4150.12849490664767627No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG3980.12323125986933772No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC3980.12323125986933772No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3960.12261200730718023No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC3940.12199275474502275No Hit
CTTGCTTCCTGTAGGAATCCTGAGAAGGGAGAAACACAGTCTGGATTATT3850.11920611821531413No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG3760.11641948168560547No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA3700.11456172399913304No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT3640.11270396631266062No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3590.11115583490726694No Hit
GTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACA3500.10836919837755828No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3470.10744031953432208No Hit
CCTGGAAGGGGTCCACGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACA3460.10713069325324334No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3450.10682106697216459No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3410.10558256184784966No Hit
CCTCACCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCC3400.1052729355667709No Hit
GTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAA3340.10341517788029848No Hit
CCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTG3320.102795925318141No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT3320.102795925318141No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT3260.10093816763166857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)