FastQCFastQC Report
Sun 21 May 2023
EGAF00007914769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321367
Sequences flagged as poor quality0
Sequence length32-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT6740.20972906365619368No Hit
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC6700.20848438078583054No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA6210.19323701562388174No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5640.1755002847212066No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5170.16087526099443938No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA5040.1568300416657591No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG5000.15558535879539592No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT4880.15185131018430642No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC4880.15185131018430642No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG4830.15029545659635246No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4800.14936194444358009No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4630.14407204224453662No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4610.14344970080935504No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4250.13224755497608653No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT4220.13131404282331416No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACGTGGACCCCTTCCAGGTGAAG4220.13131404282331416No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT4120.12820233564740624No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3960.12322360416595357No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3950.12291243344836277No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3930.1222900920131812No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3820.11886721411968248No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3780.11762253124931932No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA3770.11731136053172851No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3680.1145108240734114No Hit
ATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAG3680.1145108240734114No Hit
ATGTGGAGACGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3570.11108794617991269No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3430.1067315561336416No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3420.10642038541605081No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3400.10579804398086923No Hit
ATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGA3290.10237516608737052No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3280.10206399536977973No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3260.10144165393459814No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3240.10081931249941656No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCAC3240.10081931249941656No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC3230.10050814178182575No Hit
CTGCGTGATGAGTTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGC3230.10050814178182575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)