FastQCFastQC Report
Wed 8 May 2024
EGAF00007914769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321367
Total Bases43.4 Mbp
Sequences flagged as poor quality0
Sequence length32-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC7040.21906418518391743No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT6900.21470779513764635No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA6230.19385935705906332No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5640.1755002847212066No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5320.16554282175830126No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA5270.1639869681703473No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC5040.1568300416657591No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG5000.15558535879539592No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG4930.15340716377226038No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT4910.1527848223370788No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4820.14998428587876167No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4640.14438321296212742No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4620.14376087152694583No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4400.1369151157399484No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACGTGGACCCCTTCCAGGTGAAG4330.13473692071681287No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT4270.13286989641126812No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC4130.12851350636499703No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT4130.12851350636499703No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3970.12353477488354436No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3960.12322360416595357No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA3910.1216677505779996No Hit
ATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAG3870.12042306770763643No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3820.11886721411968248No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3810.1185560434020917No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3780.11762253124931932No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3650.11357731192063902No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3580.11139911689750348No Hit
ATGTGGAGACGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3570.11108794617991269No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3420.10642038541605081No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC3390.10548687326327844No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3370.10486453182809684No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3360.10455336111050606No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCAC3310.1029975075225521No Hit
CTGCGTGATGAGTTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGC3300.1026863368049613No Hit
ATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGA3290.10237516608737052No Hit
CCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACC3250.10113048321700735No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3240.10081931249941656No Hit
GTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGATGAGTTGCACGGTGG3220.10019697106423497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)