FastQCFastQC Report
Wed 8 May 2024
EGAF00007914772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323398
Total Bases43.2 Mbp
Sequences flagged as poor quality0
Sequence length19-142
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7650.23655062801872617No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6420.19851699763140154No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA5860.18120087322741638No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4840.1496607894915862No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT4770.14749627394108805No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA4600.1422395933184497No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG4550.1406935107823796No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4520.13976586126073753No Hit
GTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGC4380.13543683015974126No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT4370.13512761365252723No Hit
CCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTG4360.1348183971453132No Hit
CCTCACCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCC4160.12863406700103278No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC4160.12863406700103278No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC4030.1246142524072505No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3980.12306816987118041No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC3920.12121287082789628No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT3850.11904835527739813No Hit
GTACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGG3830.11842992226297008No Hit
TACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGGG3810.11781148924854205No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA3800.11750227274132802No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3770.11657462321968597No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT3750.11595619020525792No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG3700.11441010766918781No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC3690.11410089116197378No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA3670.11348245814754573No Hit
GTCCACGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC3580.11069950958261957No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG3500.10822577752490738No Hit
CCCTGAATTCGGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAG3380.10451517943833913No Hit
CTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACG3320.10265988039505501No Hit
CTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTCTCTGTTTCAG3320.10265988039505501No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3310.10235066388784098No Hit
AAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACG3300.10204144738062697No Hit
CCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGA3280.10142301436619892No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3270.1011137978589849No Hit
CCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATATTGG3250.10049536484455686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)