FastQCFastQC Report
Wed 31 May 2023
EGAF00007914772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323398
Sequences flagged as poor quality0
Sequence length19-142
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7430.2297478648600177No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6420.19851699763140154No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA5680.17563497609756398No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4710.14564097489780395No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA4550.1406935107823796No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG4540.14038429427516558No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT4530.14007507776795158No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4430.13698291269581134No Hit
CCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTG4230.13079858255153093No Hit
GTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGC4220.1304893660443169No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT4170.1289432835082468No Hit
CCTCACCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCC4010.12399581939282246No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC3910.12090365432068226No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT3850.11904835527739813No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC3850.11904835527739813No Hit
GTACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGG3830.11842992226297008No Hit
TACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGGG3810.11781148924854205No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC3800.11750227274132802No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA3780.11688383972689997No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3770.11657462321968597No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3700.11441010766918781No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC3690.11410089116197378No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT3590.11100872608983357No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG3580.11069950958261957No Hit
GTCCACGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC3480.10760734451047933No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA3430.10606126197440925No Hit
CCCTGAATTCGGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAG3380.10451517943833913No Hit
CTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTCTCTGTTTCAG3320.10265988039505501No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG3320.10265988039505501No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3310.10235066388784098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)