FastQCFastQC Report
Wed 31 May 2023
EGAF00007914777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302923
Sequences flagged as poor quality0
Sequence length20-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC7360.24296603427273597No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT7030.23207217675778993No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA6250.2063230589951902No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA5840.19278826632510573No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG5550.1832148763877289No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5450.17991370744380586No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5450.17991370744380586No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT5360.17694265539427512No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4750.15680552483634455No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA4400.1452514335326139No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4140.13666839427841398No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4110.13567804359523708No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG4080.13468769291206015No Hit
GTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTTC4070.13435757601766787No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG4040.13336722533449094No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3920.1294058226017833No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3900.12874558881299866No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3810.12577453676346795No Hit
CTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGACATGC3780.12478418608029103No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC3710.1224733678195449No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT3710.1224733678195449No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3700.12214325092515259No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT3620.11950231577001418No Hit
ATGTGGAGATGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3530.11653126372048342No Hit
CTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACAT3520.11620114682609113No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT3510.11587102993169882No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3490.11521079614291421No Hit
TGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCATG3490.11521079614291421No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3410.11256986098777577No Hit
CTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGTGGAGATGAGC3410.11256986098777577No Hit
CGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTT3350.11058915962142193No Hit
CTTTGCGATCTGCACACACCAGTTGAGCAGGTACTGGGAGCCAATATTGT3310.10926869204385273No Hit
ATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAG3270.10794822446628351No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3270.10794822446628351No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3240.1069578737831066No Hit
CTGGTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTT3240.1069578737831066No Hit
CTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAGG3220.10629763999432199No Hit
CTTGATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTC3210.10596752309992968No Hit
TTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGC3200.10563740620553738No Hit
CAGCTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTG3130.10332658794479126No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3120.10299647105039894No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3120.10299647105039894No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT3120.10299647105039894No Hit
GTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAATTCA3120.10299647105039894No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3090.10200612036722204No Hit
CTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCC3080.10167600347282973No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCAC3060.10101576968404513No Hit
CATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCT3050.10068565278965282No Hit
CCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACAT3050.10068565278965282No Hit
CTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGT3030.10002541900086821No Hit
CTGCGTGATGAGCTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGC3030.10002541900086821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)