FastQCFastQC Report
Wed 8 May 2024
EGAF00007914777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302923
Total Bases39.9 Mbp
Sequences flagged as poor quality0
Sequence length20-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC7950.26244293104188193No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT7370.2432961511671283No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA6330.20896399415032862No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA6260.2066531758895825No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG5740.1894870973811827No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5730.18915698048679036No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5450.17991370744380586No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT5370.17727277228866742No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4800.1584561093083061No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA4710.15548505725877532No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4360.14393096595504468No Hit
GTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTTC4330.14294061527186777No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG4280.14129003079990624No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4130.13633827738402168No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG4050.13369734222888327No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC4000.13204675775692173No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3980.1313865239681371No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3960.1307262901793525No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC3950.13039617328496023No Hit
CTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGACATGC3950.13039617328496023No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3900.12874558881299866No Hit
CTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACAT3800.12544441986907562No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT3740.12346371850272182No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3680.12148301713636799No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT3630.11983243266440646No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3620.11950231577001418No Hit
ATGTGGAGATGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3560.11752161440366034No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT3520.11620114682609113No Hit
ATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAG3520.11620114682609113No Hit
TGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCATG3510.11587102993169882No Hit
CTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAGG3500.1155409130373065No Hit
CGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTT3480.1148806792485219No Hit
CTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGTGGAGATGAGC3410.11256986098777577No Hit
CTTTGCGATCTGCACACACCAGTTGAGCAGGTACTGGGAGCCAATATTGT3400.11223974409338346No Hit
CTTGATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTC3370.11124939341020654No Hit
CAGCTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTG3320.10959880893824503No Hit
TTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGC3320.10959880893824503No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3290.10860845825506812No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3270.10794822446628351No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3260.1076181075718912No Hit
CTGGTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTT3260.1076181075718912No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3240.1069578737831066No Hit
CTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACCGT3240.1069578737831066No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT3240.1069578737831066No Hit
CATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCT3210.10596752309992968No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCAC3200.10563740620553738No Hit
CTCCCCACCAGACCATGAGAGGCCCTGCGGCCCAGCCCAGAGGCCTGTGC3200.10563740620553738No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC3190.10530728931114508No Hit
GTGGGCCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACT3190.10530728931114508No Hit
TTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGA3180.10497717241675278No Hit
CTGCGTGATGAGCTGCACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGC3170.10464705552236046No Hit
CTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCC3160.10431693862796816No Hit
CCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACAT3140.10365670483918356No Hit
GTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAATTCA3120.10299647105039894No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3110.10266635415600664No Hit
CTTTCGGAGATGTTGCTTCTCTTAATTCCTTGATAGCGACGGGAATTTTA3090.10200612036722204No Hit
AAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAG3080.10167600347282973No Hit
CCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCCACA3070.10134588657843742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)