FastQCFastQC Report
Wed 31 May 2023
EGAF00007914812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327839
Sequences flagged as poor quality0
Sequence length22-142
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC7740.2360914961307227No Hit
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7570.23090602399348462No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5990.1827116358944482No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG5270.16074963625438096No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA5100.15556416411714286No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4770.1454982476154454No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA4740.14458316429710924No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC4620.1409228310237647No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC4480.13665244220486275No Hit
CTTCACAGCCCTGCGTAAACGTCCCTGTGCTAGGTCTTTTGCAGGCACAG4390.13390719224985437No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC4320.1317719978404034No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT4310.13146697006762464No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG4300.13116194229484596No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC4240.12933177565817366No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC4170.12719658124872268No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT4070.12414630352093557No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA4050.12353624797537817No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3950.12048597024759106No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG3910.1192658591564762No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA3850.11743569251980393No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC3800.11591055365591037No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3710.11316530370090196No Hit
GTACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGG3640.11103010929145098No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA3640.11103010929145098No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG3550.1082848593364426No Hit
GTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAA3500.10675972047254903No Hit
GTACTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTC3480.1061496649269916No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG3470.10584463715421288No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3390.1034044149719832No Hit
CCTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACC3380.10309938719920447No Hit
GTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACA3360.10248933165364707No Hit
AACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCT3350.10218430388086837No Hit
TACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGAAGGG3350.10218430388086837No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3330.10157424833531092No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT3310.10096419278975349No Hit
CCCTGAATTCGGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAG3300.10065916501697479No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT3290.10035413724419609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)