FastQCFastQC Report
Sun 21 May 2023
EGAF00007914814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466570
Sequences flagged as poor quality0
Sequence length20-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC10080.21604475212722635No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT8440.1808946138843046No Hit
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC8320.17832265254945667No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG6190.13267033885590587No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC5990.12838373663115932No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC5770.1236684741839381No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA5750.12323981396146345No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG5560.11916754184795421No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG5560.11916754184795421No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA5420.11616692029063162No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC5240.11230897828835973No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC5240.11230897828835973No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG5120.1097370169535118No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA5090.10909402661979982No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC5020.10759371584113853No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT4870.1043787641725786No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG4780.10244979317144266No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT4770.10223546306020533No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA4760.10202113294896799No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA4680.10030649205906939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)