FastQCFastQC Report
Wed 31 May 2023
EGAF00007914827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316412
Sequences flagged as poor quality0
Sequence length20-142
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC5930.1874138781082892No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5510.17414004525744914No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG5220.1649747797175834No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT4990.15770577601355196No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4980.15738973237424625No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA4790.15138490322743764No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA4560.1441158995234062No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4520.14285172496618334No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG4330.13684689581937473No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4280.13526667762284617No Hit
TATAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTG4090.12926184847603756No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT4050.1279976739188147No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3940.12452119388645184No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC3890.12294097568992327No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT3870.12230888841131185No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG3730.11788427746103182No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA3620.11440779742866895No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG3550.11219549195352893No Hit
CATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTA3550.11219549195352893No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3410.10777088100324894No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3360.10619066280672035No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3330.10524253188880321No Hit
CCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACC3320.10492648824949749No Hit
CCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTC3310.10461044461019177No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3300.10429440097088605No Hit
CTTCTCTTAATTCCTTGATAGCGACGGGAATTTTAACTTTCTCACCTTCT3230.10208209549574605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)