FastQCFastQC Report
Sun 21 May 2023
EGAF00007914828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334740
Sequences flagged as poor quality0
Sequence length20-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC7680.2294317978132282No Hit
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7540.22524944733225788No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5410.16161797215749538No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC5110.15265579255541614No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA4950.14787596343430723No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4870.14548604887375274No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA4750.14190117703292107No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG4670.13951126247236662No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC4430.13234151879070322No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG4110.1227818605484854No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC4020.12009320666786162No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT4020.12009320666786162No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC3980.11889824938758439No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC3880.11591085618689131No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT3870.11561211686682202No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3860.1153133775467527No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3840.1147158989066141No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3780.11292346298619825No Hit
CTTGCTTCCTGTAGGAATCCTGAGAAGGGAGAAACACAGTCTGGATTATT3670.10963733046543588No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA3670.10963733046543588No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT3660.10933859114536655No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA3540.10575371930453487No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG3530.10545497998446554No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG3480.10396128338411902No Hit
AACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCT3440.10276632610384179No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG3440.10276632610384179No Hit
GTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAA3430.10246758678377248No Hit
CTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAG3420.10216884746370317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)