FastQCFastQC Report
Wed 8 May 2024
EGAF00007914829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332943
Total Bases45 Mbp
Sequences flagged as poor quality0
Sequence length20-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC6880.20664197775595222No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT6730.2021367020781335No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA6700.20123564694256973No Hit
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5990.17991067540089445No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA5420.1627906278251833No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT5220.15678359358809166No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA5220.15678359358809166No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG5210.15648324187623708No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG5110.15347972475769125No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC4870.1462712836731813No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA4830.14506987682576297No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4810.1444691734020538No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4810.1444691734020538No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG4630.13906284258867133No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG4540.13635967718198008No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC4430.1330558083515797No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC4430.1330558083515797No Hit
TCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATAT4230.1270487741144881No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT4150.12464596041965141No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT4060.12194279501296017No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3980.11953998131812352No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC3880.1165364641995777No Hit
CTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACA3860.11593576077586853No Hit
GTTGAGCAGGTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGT3850.11563540906401396No Hit
TGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCATG3740.11233154023361357No Hit
TGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTATAAGGCC3700.11113013338619523No Hit
GTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACCTAA3690.11082978167434064No Hit
CCCAGCAGGCGGCACACGTGGGGGTTGTCCACGCTGGCCATCACGTAGGC3600.10812661626764943No Hit
CACGGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGT3520.10572380257281276No Hit
ATGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAG3510.10542345086095818No Hit
ATGTGGAGATGAGCAGGGTCTAGAGCAGAGCAGCTGCCAGACATGAGAAA3510.10542345086095818No Hit
ATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGA3500.1051230991491036No Hit
CTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGTGGAG3490.10482274743724902No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3470.10422204401353985No Hit
ATAGCGACGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTA3470.10422204401353985No Hit
CTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGACATGC3470.10422204401353985No Hit
CATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTA3470.10422204401353985No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3410.10241993374241237No Hit
CTGGTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTT3400.10211958203055779No Hit
CCCTGCGGCCCAGCCCAGAGGCCTGTGCCAGGGACCTTACCTTATACACC3370.10121852689499403No Hit
GTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTTTC3370.10121852689499403No Hit
GTTCCTGGCTGCCAGGTCGCGGTGCACCAAGCGACGGTCCTCCAAGTAGT3340.10031747175943029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)