FastQCFastQC Report
Wed 8 May 2024
EGAF00007914833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280720
Total Bases38.2 Mbp
Sequences flagged as poor quality0
Sequence length22-142
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAG5370.19129381590196637No Hit
TATAGTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTG5360.19093758905671132No Hit
CTTGCAAGGACTCTGGGCTCCCCACCAGACCATGAGAGGCCCTGCGGCCC5280.18808777429467086No Hit
CCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGTT4820.17170133941293816No Hit
TAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTT4500.16030208036477628No Hit
CCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCC4330.1542462239954403No Hit
GTCACATTTTCATTATTTTTATTATAAGGCCTGCTGAAAATGACTGAATA4020.14320319179253346No Hit
TATAACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTG4000.14249073810202337No Hit
ACACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTC3940.140353377030493No Hit
CTGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCA3940.140353377030493No Hit
CTTGGGAAAAACACTGGAGTTTCCCAAACACTCAGTGAAACAAAGAGTAA3720.13251638643488176No Hit
CTTCGCATGGTGGCCAGAAGGAGGGGCACATGGACCCCTTCCAGGTGAAG3690.13144770589911656No Hit
CCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCG3650.1300227985180963No Hit
CTTTGCCTCCTTCTGCATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTG3620.12895411798233117No Hit
CTTATGTGTGACATGTTCTAATATAGTCACATTTTCATTATTTTTATTAT3500.12467939583927046No Hit
CCCACTAGAGCTAGAAAGGGAAAGACATAGAAAGTGAACATTTAGGATGT3490.12432316899401538No Hit
CACCTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCT3450.12289826161299515No Hit
CCCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTC3420.12182958107722999No Hit
GTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACATCGAGGA3290.11719863208891422No Hit
CTAGTGGGAAGGCAGCCTGGTCCCTGGTGTCAGGAAAATGCTGGCTGACC3180.11328013679110858No Hit
CCTGAGGTTCAGAGCCATGGACCCCCACACAGCAAAGCAGAAACTCACAT3180.11328013679110858No Hit
CTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGCAGC3100.11043032202906812No Hit
TGCGGTGTTTTCACCAGTACGTTCCTGGCTGCCAGGTCGCGGTGCACCAA3100.11043032202906812No Hit
CATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTA3080.109717868338558No Hit
TATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGAT3070.10936164149330294No Hit
GTGGAGTATTTGATAGTGTATTAACCTTATGTGTGACATGTTCTAATATA3040.10829296095753776No Hit
ACCGTGCCGAACGCACCGGAGCCCAGCACTTTGATCTTTTTGAATTCAGT3000.10686805357651753No Hit
CTTCTCCACTGGGTGTAAGAGGCTCCACAAGCTGGGGGGGACAAGAACAC2990.10651182673126246No Hit
CTTCTCTTAATTCCTTGATAGCGACGGGAATTTTAACTTTCTCACCTTCT2970.10579937304075235No Hit
CTTTTTTGAAGTAAAAGGTGCACTGTAATAATCCAGACTGTGTTTCTCCC2970.10579937304075235No Hit
CATGGTATTCTTTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCT2910.10366201196922199No Hit
GTGCAGGACCATTCTTTGATACAGATAAAGGTTTCTCTGACCATTTTCAT2890.10294955827871187No Hit
CTGCCAGACATGAGAAAAGGTGGGCCTGAGGTTCAGAGCCATGGACCCCC2850.10152465089769164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)