FastQCFastQC Report
Wed 8 May 2024
EGAF00007914842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337355
Total Bases45 Mbp
Sequences flagged as poor quality0
Sequence length21-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC7420.21994634731958917No Hit
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC6980.20690370677772674No Hit
CTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGA5890.17459352907174933No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT5820.1725185635309985No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA5810.17222213988231982No Hit
CCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAAC5190.15384387366424093No Hit
GTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCA4980.1476189770419884No Hit
GTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGG4860.14406189325784413No Hit
CCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAA4630.1372441493382342No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG4620.13694772568955552No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG4580.13576203109484075No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT4570.13546560744616207No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC4540.13457633650012596No Hit
ACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGG4350.12894428717523085No Hit
GTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCTTCCCACTAGC4290.1271657452831587No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT4260.1262764743371226No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC4220.12509077974240784No Hit
TTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCATGTACTGGTC4220.12509077974240784No Hit
CTTGCTTCCTGTAGGAATCCTGAGAAGGGAGAAACACAGTCTGGATTATT4020.119162306768834No Hit
ATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATA4010.11886588312015534No Hit
ATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAG3960.11738376487676187No Hit
ATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAATG3910.11590164663336841No Hit
CCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTG3890.11530879933601103No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG3890.11530879933601103No Hit
TTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTT3860.11441952838997496No Hit
AATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTATT3800.1126409864979028No Hit
AGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTG3800.1126409864979028No Hit
TTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGA3790.11234456284922412No Hit
GTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAA3750.11115886825450935No Hit
CTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACAGGCCT3640.10789820811904374No Hit
CTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTT3540.10493397163225682No Hit
CCTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACC3530.10463754798357813No Hit
AACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCT3520.10434112433489943No Hit
CCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCCTCTCCCTCC3520.10434112433489943No Hit
CCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAACACCGCAGCATGTCA3520.10434112433489943No Hit
CTCCACAGCCCCAGTGTCCCTCACCTTCGGGGTGCATCGCTGGTAACATC3510.10404470068622075No Hit
GTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACA3510.10404470068622075No Hit
TAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGT3460.10256258244282729No Hit
CCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGA3400.10078404055075514No Hit
CCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGAT3400.10078404055075514No Hit
CTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGG3400.10078404055075514No Hit

[OK]Adapter Content

Adapter graph