FastQCFastQC Report
Wed 8 May 2024
EGAF00007914844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007914844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250339
Total Bases34.7 Mbp
Sequences flagged as poor quality0
Sequence length19-142
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAACCCAAGGTACATTTCAGATAACTTAACTTTCAGCATAATTATC4140.16537575048234593No Hit
CTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTAC3140.12542991703250392No Hit
GTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGGGACCAGGCTGCCT3100.12383208369451024No Hit
GTCCATGTGCCCCTCCTTCTGGCCACCATGCGAAGCCACACTGACGTGCC2940.11744075034253551No Hit
CTCATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTG2880.115044000335545No Hit
CATGGCATTAGCAAAGACTCAAAAAATAAAAACTATAATTACTCCTTAAT2860.11424508366654816No Hit
CATGAAAATGGTCAGAGAAACCTTTATCTGTATCAAAGAATGGTCCTGCA2630.10505754197308449No Hit
CCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAGCCCAGAG2600.10385916696958924No Hit
ACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAA2550.10186187529709714No Hit
GTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGG2530.1010629586281003No Hit
GTCCATGGCTCTGAACCTCAGGCCCACCTTTTCTCATGTCTGGCAGCTGC2520.10066350029360187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)