FastQCFastQC Report
Mon 22 May 2023
EGAF00007916510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007916510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179391493
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT23376961.3031253382790007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA16580930.9242874186904728Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5903110.32906298405131174Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5643040.3145656410808733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACACACACACACACACACACACACACACACACACACACACACACACAC5010050.27928024435361604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACACACACACACACAC3924930.21879131135833738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA3305490.18426124587747314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGTGTGTGTGTGTGTGTG3188260.17772637635609623Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA2921350.16284774440223873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTGTGTGTGTGTGTGTGT2748400.15320681901008537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGAATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCAC2261130.12604443846175026No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA2142600.11943710173592233Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA2031690.1132545343161841No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG2018690.11252986227167418No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC1864840.10395364734491618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)