FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007917503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.2069679199724043No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA50.17247326664367024No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT40.1379786133149362No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT40.1379786133149362No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA40.1379786133149362No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC40.1379786133149362No Hit
CGGTTGTACCGTCAATGGTTCGTCACTATGAACATTGGTCTCATGGGGGC40.1379786133149362No Hit
GCCATGTACTCGAACAGTTTCGTCGGCAACGACGCGACATAGTTCGGCAG40.1379786133149362No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30.10348395998620215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCTATTGCTATCTATAAATAAATGGCAACAGGAAATGTTTCACAAGGG30.10348395998620215No Hit
GGATTCCACTGAGCCACCCTACAGCCAGAAGAGATATGAGGAAATTGTTA30.10348395998620215No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG30.10348395998620215No Hit
GGATGAGCACCGGCTTTTCCGCATTCTGCATCAGGGCGATTAGCTGTCTC30.10348395998620215No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT30.10348395998620215No Hit
CAACATGGCAGTTGGCCCACATGGAATATCGGTTTGAGGCCCCGAGCTGG30.10348395998620215No Hit
ATACAAGGCACGACCTGATCGCACGATCCGGTCGAGAGCTCCCATTGTCT30.10348395998620215No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA30.10348395998620215No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC30.10348395998620215No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC30.10348395998620215No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT30.10348395998620215No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG30.10348395998620215No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT30.10348395998620215No Hit
CTCTGTCACCTGGGGTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAC30.10348395998620215No Hit
CGCCGGGATTGGTGTCGGCATGTTCGGAGGCGCTGGTTACTTCGGTATCG30.10348395998620215No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30.10348395998620215No Hit
CCATAGGAATTGACGTTTTGACCCAGTAACGTAATCTCCTGGACTCCCTC30.10348395998620215No Hit
CTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTA30.10348395998620215No Hit
GATGCGACGGCACGTTCAATGGCTTCAAGCTGCTCGAATTCGCAAGAGCG30.10348395998620215No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30.10348395998620215No Hit
GCATCCGGCCGGGCGTGCCGCTTGAAAAACAGTCCCTTGCTGACCTGCGC30.10348395998620215No Hit
TCCTTGGAGGGGGCCTCGGCGCATGCGCTCCAGTGGCCCTTGACCTGCCA30.10348395998620215No Hit
AGGAGATGGAGTGCAATTGTGGTGTCCATCATTATGACACTGTCTCTTAT30.10348395998620215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)