FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007917509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400244
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.30231558749163007No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10990.27458250467215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10370.25909195390811607No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9140.22836069997301647Clontech Universal Primer Mix Long (96% over 26bp)
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8210.20512487382696554No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8130.20312609308321922No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT7970.19912853159572663No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7800.19488112251526568No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6500.16240093542938808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.13991465206224205No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA5500.13741617613255913No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG5480.13691648094662257No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5260.1314198339013202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.1314198339013202No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5070.12667272963492268Clontech Universal Primer Mix Long (96% over 25bp)
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC4930.12317486333336665No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG4510.1126812644286985No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4500.1124314168357302No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4490.11218156924276192No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG4460.11143202646385704No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT4410.1101827884990156No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT4370.10918339812714245No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4360.10893355053417417No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT4240.10593537941855469No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4230.10568553182558639No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC4200.10493598904668153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)