FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007917582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069234
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1369216.616989668640666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC884114.272643886578319No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT868354.196480436721995Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG804063.8857857545352528No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG621793.004928393792099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG431522.085409383375684Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG401971.9426029148950772Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT357111.7258077143522677Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC272451.3166708066849857Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC260091.256938557939798Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT233561.128726862210847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC200780.9703107526746613Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT172450.8334001857692267Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC145140.7014189791971328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG135340.6540584583473884Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC132920.6423633093212271Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC120020.5800213992230941Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT119260.5763485425041344Clontech SMART CDS Primer II A (100% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG119220.5761552342557681Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG109950.5313560476968772Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA103280.499121897281796Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA102980.4976720854190488No Hit
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC102120.4935159580791733Clontech SMART CDS Primer II A (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG101200.48906986836674826Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA91820.4437390841248501Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA81480.3937689019221606No Hit
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG79690.3851183578077685No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC76700.37066856624238725Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT75170.3632745257423762Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGG73960.35742695122929546No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC71980.34785819293516346Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG71260.34437864446457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG70770.34201061842208275Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG68860.3327801495625918No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA68500.331040375327295No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC64270.31059802806255843Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC62710.3030590063762726Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTAC59620.2881259441899756No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA59520.2876426735690598Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA57140.2761408327912648Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA52920.25574681258861975Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAA50470.24390668237618368No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA47530.22969852612126032Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC46640.22539741759511006Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGTTTGTTAG45240.21863162890228943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42240.2041335102748167Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT42040.20316696903298517No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTC41620.20113723242513895No Hit
GGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41200.19910749581729278No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTC39200.18944208339897758No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT39180.18934542927479442Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTCTCTTAT38990.1884272150950545No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT36490.17634544957216053Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT36440.17610381426170263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTCTCTTAT36410.1759588330754279No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT36300.17542723539242058Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAA35010.1691930443826073No Hit
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC34140.16498858998064017No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT30850.1490889865525117Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC30660.14817077237277176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA29480.1424681790459658Clontech Universal Primer Mix Long (96% over 26bp)
CCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT29380.14198490842505004Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC29080.14053509656230276Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA28480.13763547283680821Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.13657227747079353No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG26550.12830834985313405Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT24340.11762806913089578Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT23980.11588829489559906Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22730.10984741213415206Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAG22120.10689946134656593No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTCTCTTATACACAT21410.10346823993806405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)