FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007917732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798697
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1112473.9749569174512285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC1040153.7165509521037823No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG1024843.6618469237648803No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT578982.068748421140266Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG487501.7418820258141559Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG346831.239255267719228Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG322261.1514644136181946Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC210000.7503491803507132Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT199490.7127959904198276Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT182020.6503740847973182Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151520.5413947990797147Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT137770.49226479322341793Clontech Universal Primer Mix Long (100% over 23bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC126200.45092412647742863Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC113610.4059389065697358Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG97520.34844786698953123Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGG86330.3084649749508432No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT83290.2976027772924329Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG83190.2972454681589325Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA82480.29470857331108014No Hit
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC79710.28481111031312073Clontech SMART CDS Primer II A (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG78110.27909416417711524Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC77940.2784867386501647Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC77750.2778078512965141Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGAT76420.27305563982095954Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA76060.27176932694035827Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG71630.25594053232629327Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTAC70820.2530463283449405No Hit
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG70740.25276048103814025No Hit
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG67540.2413265887661294No Hit
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA60360.215671792980805No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG58420.2087399957908984Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA55170.19712744895213738No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA54660.19530517237128564Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54410.19441189953753477Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA46850.16739932904490912Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC46800.16722067447815894Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC46690.16682763443130857Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT44130.15768052061369986Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA43200.1543575456721467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC43120.15407169836534643Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTC42830.15303550187819548No Hit
GCTTCCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGT40320.14406704262733694Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTC40230.14374546440718663No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTCTCTTAT39890.14253061335328548No Hit
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT39390.1407440676857838No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC39260.14027956581223333Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAA39020.13942202389183253No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGTTTGTTAG38640.13806424918453125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC37910.13545589250997875No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTCTCTTAT35530.12695193513267067No Hit
CCCATGTACTCTGCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGCT35490.1268090114792705Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC34300.1225570327906165Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAA31540.11269530070600713No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA31300.1118377587856063Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30990.11073010047175524No Hit
GGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30380.10855051475740318No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA28850.10308368501484799Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)