FastQCFastQC Report
Wed 8 May 2024
EGAF00007917733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798697
Total Bases419.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1104693.9471582668649017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG1047043.741169551401956No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC1035613.7003291174428674No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT711062.5406823246675145Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG474091.6939668710117601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG347011.2398984241595286Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG318571.138279706592032Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT229610.8204175014301297Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC203490.7270883557598411Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC181240.6475870735560155Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT178380.6373680323379058Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC149860.535463467463609Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT133870.4783297370169046Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC111580.3986855311596789Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG97520.34844786698953123Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC93800.3351559672233186Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA90460.32322184216440725No Hit
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC89540.31993459813620406Clontech SMART CDS Primer II A (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT88340.31564688853420003Clontech SMART CDS Primer II A (100% over 26bp)
GGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGG86760.31000140422489464No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG83920.29985382483348505Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG81400.29084963466927644Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG76050.2717335960270083Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC74660.26676699907135354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA74270.26537349345070227Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG72760.2599781255348471No Hit
ACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTAC71410.25515445223259253No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA66070.2360741445036744Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG64710.23121474028806976No Hit
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA61160.2185302660488077No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56320.20123650398739126Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA53940.19273254661008318No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC53890.19255389204333304Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53760.1920893901697826Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC52760.18851629883477916Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT51450.18383554918592473Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC47400.16936452927916099Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA45770.16354039040310545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA44330.15839513888070056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC43690.1561083604262984Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTC43550.1556081276393979No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTC41790.14931948688979194No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGTTTGTTAG41150.14703270843538976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT40800.14578212646813857No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTCTCTTAT40310.14403131171398692No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAA40210.14367400258048654No Hit
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC39650.14167307143288466No Hit
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC39150.13988652576538296Clontech SMART CDS Primer II A (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTCTCTTAT36800.13148976112812497No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36740.13127537564802477Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA34670.12387907658456775Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.11030132951155484No Hit
GTATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAA30800.11005121311810459No Hit
GGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28620.10226187400779721No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT28200.10076117564709577Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT28090.1003681356002454Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)