FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007917757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007917757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500377
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA951593.8057860874580114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG761683.046260623897916No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC750082.9998676199629095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT572712.290494593415313Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG413681.654470505847718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG300251.2008189165073906Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG278781.114951865258719Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT230570.9221409411460753Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC175750.7028940035842595Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT157730.6308248716093613Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC154470.6177868377448681Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC123440.4936855522187254Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT114190.4566911309774486Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC95890.3835021678730847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG87690.35070711336730426Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT87110.34838746317055386Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC80880.32347122053994254Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC76920.30763360885178515Clontech Universal Primer Mix Long (96% over 25bp)
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC75060.30019473063462027Clontech SMART CDS Primer II A (95% over 21bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA72940.29171600922580876No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG72440.28971631078033433Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70150.28055769190006147Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA69190.2767182708847506Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGG67360.26939937457431423No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG66650.2665598027817405Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG58190.23272490508431326No Hit
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG54870.21944690740636313No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA54460.2178071546810741Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTAC53810.21520754670195735No Hit
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA49380.19749021847505396No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG47520.1900513402578891Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47030.18809163578132418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC46050.1841722268281943Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA44620.17845308927413747No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT44290.17713328830012434Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC43740.17493362001010246Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC43390.1735338310982704Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC38280.15309691298552178Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA37630.15049730500640504Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA36810.14721779955582698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGTTTGTTAG35270.14105872834376576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAA33660.13461969934933812No Hit
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC32910.13162015168112648Clontech SMART CDS Primer II A (96% over 26bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT32190.12874058591964332No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA32120.1284606281372769Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.128140676386001No Hit
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC28690.11474269680132236No Hit
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCCTGTC28620.11446273901895593No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGCTGTC27950.11178314310202021No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27410.10962346878090783Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA26940.10774375224216189Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAA25430.10170466293682912No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT25290.10114474737209628Clontech SMART CDS Primer II A (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)