FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758659
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT167710.4461963695030595No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG151690.4035747855817727No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCTAAGG144720.38503093789566967No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA121180.32240221845078254No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA119810.3187573014737437No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT110460.2938814082362885No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG96020.2554634511936305No Hit
GTATTCCAGCGCCTGGATGTGGGTTTTGCGCGGCAACGCAACTGAAAGAG87420.23258295046185354No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA86460.2300288480545854No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC75280.20028419710327539No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTTGGGAAAAGGA74720.198794304032369No Hit
ATCTATCACCCTGGAAACAGTTTGTCTGGAGATAGGGTTTAACGGTCGGA71360.18985494560693056No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG70950.1887641310371598No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAG69090.1838155576230778No Hit
CCCTTATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCT67570.17977156214490328No Hit
ACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT65200.17346612182696008No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACG60510.16098826735811894No Hit
GTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG60390.16066900455721042No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTG59330.15784884981585187No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG56740.15095809436290974No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG55800.14845720242245972No Hit
ATCCTAAGCCATAGGGAAGCTCCGTTTCAAAGTGGGCACTTGTGCCCCCC53910.1434288133081506No Hit
CCCTTAGAGCCAATCCTTTTCCCAAAGTTACGGATCTATTTTGCCGACTT53090.14124718416860907No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT51810.13784171429225156No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT50140.1333986403129414No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC49910.13278671994453342No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT49860.1326536937774882No Hit
GTGATAGATCGTTAAGAAGAAAAGATAACTCTGCTTGGGGCCCCTGCCGC49830.13257387807726106No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT49810.13252066761044298No Hit
GAGTAATACGTTCCATCGGTTTTTCAAGGACTGTTAAGGACGCACCGGAT48920.13015280183703815No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCT48840.12993995996976582No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTTGGGAAAAGGATTGG47930.1275188837295429No Hit
GCCCATACCCCCCCTTCAGGGTAGAAACGATGCCCTGAAGAGTAGGCGGC47910.12746567326272482No Hit
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAG46970.12496478132227479No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG46350.12331525685091412No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAG46180.12286296788296039No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT44290.11783457876865128No Hit
GAGTACGACCTGGCGTGAGAATTATTCCCTCCAGAGGATTTTCAAGGGAC44200.11759513166796987No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC43590.11597221243001825No Hit
CCCTTGACGTCCCTTGAAAATCCTCTGGAGGGAATAATTCTCACGCCAGG43310.11522726589456506No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA42650.1134713204895682No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGC42480.1130190315216145No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG42420.11285940012116023No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG41390.11011906108002881No Hit
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG40890.10878879940957667No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT40160.10684661737071653No Hit
GCCATAGGGAAGCTCCGTTTCAAAGTGGGCACTTGTGCCCCCCTCGGCGA39810.10591543420140001No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCG39570.10527690859958298No Hit
GGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAAC39290.10453196206412979No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAG39080.10397325216253989No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGA37720.10035494041891005No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)