FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758659
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT163700.4355276709060332No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG145050.3859089105981681No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCTAAGG134960.3590642300884438No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA117660.31303717629079947No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA114290.30407121263195197No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT102640.27307611571041696No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG94880.25243045458499963No Hit
GTATTCCAGCGCCTGGATGTGGGTTTTGCGCGGCAACGCAACTGAAAGAG82520.2195463860914225No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA79200.21071344859962027No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTTGGGAAAAGGA77580.20640340078735528No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC73510.1955750707898748No Hit
ATCTATCACCCTGGAAACAGTTTGTCTGGAGATAGGGTTTAACGGTCGGA71250.1895622880394311No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG67060.1784146952410421No Hit
CCCTTATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCT67030.17833487954081495No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAG66380.17660553936922715No Hit
ACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT61040.16239834472879824No Hit
GTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG57810.15380485433767735No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTG57690.15348559153676883No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACG57180.15212872463290764No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG55940.14882967569018632No Hit
GTGATAGATCGTTAAGAAGAAAAGATAACTCTGCTTGGGGCCCCTGCCGC53820.1431893662074692No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG53270.14172607836997184No Hit
ATCCTAAGCCATAGGGAAGCTCCGTTTCAAAGTGGGCACTTGTGCCCCCC52180.13882610792838615No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT51730.13762887242497923No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC49970.13294635134498767No Hit
CCCTTAGAGCCAATCCTTTTCCCAAAGTTACGGATCTATTTTGCCGACTT49780.13244085191021585No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT48190.12821061979817802No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT47990.12767851512999717No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTTGGGAAAAGGATTGG47970.1276253046631791No Hit
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCGAAGGCAGCCTAAG46890.12475193945500243No Hit
GAGTAATACGTTCCATCGGTTTTTCAAGGACTGTTAAGGACGCACCGGAT46750.12437946618727583No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT46420.12350149348477743No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCT46310.12320883591727795No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAG45060.11988318174114758No Hit
GCCCATACCCCCCCTTCAGGGTAGAAACGATGCCCTGAAGAGTAGGCGGC44240.11770155260160606No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG43450.11559973916229166No Hit
CCCTTGACGTCCCTTGAAAATCCTCTGGAGGGAATAATTCTCACGCCAGG43330.11528047636138314No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA43230.1150144240272927No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGC43120.11472176645979323No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC42440.11291261058797833No Hit
GAATAATTCTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG42280.11248692685343364No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT41360.11003924537980167No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG40810.10857595754230431No Hit
GAGTACGACCTGGCGTGAGAATTATTCCCTCCAGAGGATTTTCAAGGGAC40710.10830990520821389No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAG40580.10796403717389634No Hit
GTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGG40550.10788422147366919No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTTGGGAA38370.10208428059049783No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT38250.10176501778958932No Hit
GGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAAC38090.10133933405504464No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCG37990.10107328172095421No Hit
GCCATAGGGAAGCTCCGTTTCAAAGTGGGCACTTGTGCCCCCCTCGGCGA37670.10022191425186483No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)