FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153380
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA142750.23198632296396451No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA139740.2270947024237086No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT132820.2158488505504292No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG130930.21277736788561735No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT130650.2123223334167563No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA128380.20863330397277594No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA114810.1865803834640474No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA113480.18441896973695757No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA103390.16802147762693026No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG100210.16285358615915155No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT100070.16262606892472106No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG99310.161390975366384No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA93830.1524853007615327No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT90730.147447419142No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC89950.14617982312160147No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC88720.14418092170481914No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA86300.14024812379537752No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC84600.1374854145201499No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC81930.1331463358349395No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA80930.13152121273186443No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT80680.13111493195609566No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT79650.12944105515992835No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC78480.12753966112933054No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC78040.12682460696397752No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC77740.126337070033055No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG77420.125817030640071No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG76260.12393188784050391No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC76220.12386688291638091No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA75580.12282680413041287No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC72280.11746389789026518No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC71350.1159525334044054No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG70860.1151562230838986No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG70690.11487995215637584No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC70100.11392112952556155No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT69470.11289730197062428No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT67550.10977706561272017No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG66760.10849321836129087No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA66130.10746939080635359No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT65180.10592552385843226No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT64980.10560049923781727No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG64440.10472293276215672No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG64420.10469043030009524No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG64200.10433290321741871No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT63780.10365035151412719No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG63770.10363410028309646No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC63220.10274028257640518No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG63140.10261027272815916No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA63120.10257777026609766No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)