FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403676
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26890.6661282810967211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.32749036355889377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.27695478552106145No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10430.2583755288895055No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9810.24301667674075247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9280.22988733538778622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8380.20759222742991906No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7530.18653573658082226No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7290.1805903744587243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7250.179599480771708No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7220.17885631050644576No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7070.17514045918013454No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6930.17167233127557746No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6770.16770875652751216Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6730.16671786284049583No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6280.15557030886156226No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6190.15334079806577552No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6080.15061584042648063No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6070.15036811700472655No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6010.1488817764742021No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA5900.14615681883490722No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5730.14194552066508784No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT5440.13476154143421953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5410.13401837116895726No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5350.1325320306384328No Hit
GTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAA5320.13178886037317056No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC5170.12807300904685937No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA4710.11667773164617168No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA4670.11568683795915537No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG4660.11543911453740129No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC4540.11246643347635231No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG4510.11172326321109008No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4480.11098009294582784No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4340.10751196504127072No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA4260.10553017766723807No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4260.10553017766723807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4120.10206204976268096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)