FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403676
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.6621647063486559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.31188378798838673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.2801751900038645No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9550.23657586777514641No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9360.2318691227618189No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9220.22840099485726176No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8400.20808767427342723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7640.18926069422011713No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7150.1771222465541672No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6830.16919509705803665No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.1585429899226112No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6350.1573043728138408No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6340.15705664939208674No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6260.1550748620180541No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6250.1548271385963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6180.15309307464402144Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6140.15210218095700512No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6060.1501203935829725No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6030.14937722331771025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5990.14838632963069393No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5970.14789088278718576No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5790.14343186119561233No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA5720.14169779724333376No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5500.136247881964744No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT5430.13451381801246545No Hit
GTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAA5260.13030251984264607No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG4950.1226230937682696No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA4870.12064130639423695No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4440.10998919925881152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4290.10627334793250033No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC4150.10280522002794319No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA4080.10107115607566465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)