FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735221
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAAACAGAAGGCTTTCTCTCAAGGCCAAGCCCAGTCCCCATGTGCA192790.40714044814381417No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA119430.25221631683083007No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA115850.24465595164407322No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT109170.2305489015190632No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG104570.22083446580423594No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA104170.21998973226381618No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT98750.20854359279112844No Hit
GTCCTGGGGGTCCCGGAGGCGGCGCTCAGCGGTACTTCTCGGTCAGGACA93350.19713968999546166No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA91910.19409864924995054No Hit
CCTTGGAGCCGTGCGCGCGCAACTGCGCGGACCCCGGGTGCAGGTCGAAG87930.18569355052277392No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA86660.18301152153194117No Hit
GGATGTAGGCGTGCAGCTCGCTCAGCTTGGACAGGGCGCCGCCGATGTCG86640.18296928485492017No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA85240.18001271746345104No Hit
GTCCTGAGCAGGCCCAACTCCAGTGCAGCTGCCCACCCTGCCGCCATGTC81540.17219893221456822No Hit
TCACTAGCCTGTGGAGCAAGATGAATGTGGAAGAGGCTGGAGGTGAAGCC81250.1715865003977639No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG79770.1684609862982108No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT77410.1634770584097342No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG72780.15369926767937547No Hit
GGACAGAGGATACGACCGATAGGAACTTGTCCCAGGCGGCGTGGGCCTCG72580.15327690090916557No Hit
GGATACGACCGATAGGAACTTGTCCCAGGCGGCGTGGGCCTCGGCCGTGA71440.15086941031896928No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG71220.1504048068717384No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC71000.14994020342450753No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG68830.14535752396773033No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG68050.1437102935639118No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA67970.14354134685582784No Hit
GCTCCAAGGTGGTGGCCGCCGTGGGCGACGCGGTGAAGAGCATCGACGAC67330.14218977319115622No Hit
CCTTTGGAGATGCTATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCT66930.14134503965073647No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA65280.13786051379650496No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC64600.13642446677779135No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT64520.1362555200697074No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC64450.13610769170013395No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT64430.13606545502311299No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA64090.13534743151375617No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC62520.13203185236760862No Hit
CTTCAGGGGTGAACTCCTTGCCAAAGTGAGTAGCCAGAATAATCACCATC62400.1317784323054827No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC62080.13110264547314687No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG61010.12884298325252402No Hit
GTACATGGGACATATCTGCTTCCGACACAGCTGCAATCACTAGCAAGCTC60870.12854732651337708No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC60680.1281460780816777No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC60550.12787153968104129No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT60100.12692121444806906No Hit
CTCCAAAGGAAGTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG59860.12641437432381722No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC59620.12590753419956535No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC59330.125295102382761No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC59270.12516839235169805No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC59180.1249783273051036No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA59110.12483049893553015No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC58690.12394352871808939No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG58240.12299320348511716No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC58080.12265531006894925No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA57370.12115590803470418No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG55010.1161719801462276No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA54350.114778169804535No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG54310.11469369645049302No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG54110.11427132968028313No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT53950.11393343626411523No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC53900.11382784457156275No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC53740.11348995115539484No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG52950.11182160241306584No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT52650.11118805225775101No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC52050.10992095194712137No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG51970.10975200523903741No Hit
CCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTCTCCCT51960.10973088690052692No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA51310.10835819489734481No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG51180.1080836564967084No Hit
GATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCT50990.107682408065009No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG50550.10675320117054726No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG50050.10569728424502256No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG49340.1041978822107775No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT49140.10377551544056762No Hit
ACCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA48950.10337426700886823No Hit
GTTTACCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTC48940.10335314867035772No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA48810.1030786102697213No Hit
GACCAAGACTGAGAGGACCATCATTGTGTCCATGTGGGCCAAGATCTCCA48780.10301525525418982No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC48480.10238170509887501No Hit
GGCCGACACCATCGGCACCGAGACTCTGGAGAGGCTCTTCCTCAGCCACC48460.10233946842185401No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT48280.10195933832866512No Hit
ATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCT47910.10117795980377683No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC47880.10111460478824537No Hit
TTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGA47440.10018539789378363No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)