FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735221
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAAACAGAAGGCTTTCTCTCAAGGCCAAGCCCAGTCCCCATGTGCA188020.3970670006743085No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA109310.23084455825821013No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA109270.23076008490416816No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT107640.2273177957269576No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG102990.21749776831957787No Hit
GTCCTGAGCAGGCCCAACTCCAGTGCAGCTGCCCACCCTGCCGCCATGTC98860.2087758945147439No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT95460.2015956594211759No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA93060.19652725817865735No Hit
GTCCTGGGGGTCCCGGAGGCGGCGCTCAGCGGTACTTCTCGGTCAGGACA89160.18829110615956468No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA85290.1801183091560035No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA84400.17823877702856952No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA82480.17418405603455467No Hit
GGATGTAGGCGTGCAGCTCGCTCAGCTTGGACAGGGCGCCGCCGATGTCG79480.16784855448140648No Hit
TCACTAGCCTGTGGAGCAAGATGAATGTGGAAGAGGCTGGAGGTGAAGCC79140.16713053097204966No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG77570.1638149518259021No Hit
CCTTGGAGCCGTGCGCGCGCAACTGCGCGGACCCCGGGTGCAGGTCGAAG77100.1628223899159089No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT73640.15551544479127796No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG72340.15277006078491376No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG70190.14822961800515752No Hit
GGACAGAGGATACGACCGATAGGAACTTGTCCCAGGCGGCGTGGGCCTCG69290.14632896753921307No Hit
CTTCTGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGG68890.1454842339987933No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA68500.14466061879688405No Hit
GCTCCAAGGTGGTGGCCGCCGTGGGCGACGCGGTGAAGAGCATCGACGAC67550.1426543766383871No Hit
GAATAAAGCCTATCCTTGAAAGCTCTGCATCATGGGCAGTGAGCTCAGTG66840.14115497460414203No Hit
GGATACGACCGATAGGAACTTGTCCCAGGCGGCGTGGGCCTCGGCCGTGA66840.14115497460414203No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG66770.1410071462345686No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC66240.13988787429351238No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA66200.1398034009394704No Hit
CCTTTGGAGATGCTATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCT65880.1391276141071346No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT65130.13754373871884754No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC64670.13657229514736483No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA64050.1352629581597142No Hit
GTACATGGGACATATCTGCTTCCGACACAGCTGCAATCACTAGCAAGCTC64040.1352418398212037No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA61410.12968771679294377No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG61240.12932870503826538No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC61090.12901192996060798No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC61030.128885219929545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT60650.1280827230661462No Hit
TCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACC60100.12692121444806906No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC59550.12575970582999188No Hit
CTTCAGGGGTGAACTCCTTGCCAAAGTGAGTAGCCAGAATAATCACCATC59100.12480938059701964No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC58520.12358451696341101No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG58340.1232043868702221No Hit
GAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGA58280.12307767683915916No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT58060.12261307339192827No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC57180.12075465960300481No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC56750.11984657104705355No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC56750.11984657104705355No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG56740.11982545270854306No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC55810.11786144722706712No Hit
CCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGC55470.11714342371771033No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC55110.11638316353133253No Hit
CTCCAAAGGAAGTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG54910.11596079676112266No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG54240.11454586808091954No Hit
TTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGA53740.11348995115539484No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA52680.11125140727328249No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG52630.11114581558073003No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG51620.10901286339117013No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT51520.10880168000606519No Hit
GATTTATTATTTGTATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTCT51470.10869608831351271No Hit
GTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGG51390.10852714160542877No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC51310.10835819489734481No Hit
CCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTCTCCCT51180.1080836564967084No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG50960.10761905304947751No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA50370.10637307107735838No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC50350.1063308344003374No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC50120.10584511261459602No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT50070.10573952092204356No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT49590.10472584067353984No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC49240.10398669882567256No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA48780.10301525525418982No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG48750.10295190023865833No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT48730.10290966356163735No Hit
GTTTACCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTC48700.10284630854610587No Hit
ATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCT48070.10151585321994475No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG47970.10130466983483981No Hit
ACCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGCA47740.10081894804909845No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT47690.10071335635654598No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC47460.10022763457080462No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)