FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497086
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.8706742897607256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.4397629384050245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.382227622584422No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA12150.2444245060210909No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11190.22511195245893068No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11160.22450843516011315No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11160.22450843516011315No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10910.2194791243366339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10810.2174674000072422No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA10010.20137360537210866No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT9770.19654546698156858No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8230.16556491230893647No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8170.16435787771130148No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA8040.16174263608309225No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7920.1593285668878222No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7450.14987346253968128No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7290.14665470361265454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7220.14524649658208036No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT7220.14524649658208036No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA7150.14383828955150618No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA7060.14202773765505367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6930.13941249602684444No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6610.13297497817279103No Hit
GTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAA6500.13076208141046017No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6490.13056090897752098No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC6260.12593394301992009No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6220.12512925328816343No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT6160.12392221869052839No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA5900.11869173543410999No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA5790.11647883867177912No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5710.11486945920826577No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5700.11466828677532659No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG5650.11366242461063075No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5490.11044366568360404No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5390.10843194135421236No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG5330.10722490675657732No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG5230.10521318242718565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5160.10380497539661145No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC5100.10259794079897644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)