FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3898365
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1201753.082702620201033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT659571.6919144307934224Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG514401.3195275455222895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC419301.0755791209904666No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT393611.009679699053321Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG392091.005780628545557Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG372530.9556057475377497No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG351690.9021474387339308Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC256530.6580451035241698Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC203550.5221419749048639Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT203110.5210132965999849Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC167340.42925688076924556Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT150250.3854179893365552Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC138840.3561493087486677Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG115390.29599588545454314Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC112070.2874794946086372Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT110280.2828878260501518Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC109220.28016873740657944Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG103070.2643928929179284Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA96190.24674446851436438Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG93220.23912588995643044Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC91580.23491899809279015Clontech SMART CDS Primer II A (95% over 21bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA90400.2318920880933417No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG83270.21360236919836909Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA77250.19815999784525049Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG69390.17799769903536483No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC66380.17027651335880556Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA64960.16663396064760483No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT59790.15337199056527545Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC57470.14742077768500383Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56170.14408604632967925Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55360.14200825217751545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC55340.14195694861820277Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA53470.1371600658224666No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA51800.13287621861985732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC51500.132106665230167Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG49570.1271558717564928No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA48970.1256167649771122Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA42720.10958440269189776Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC42480.10896875998014552Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40050.10273537752365416Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT39160.1004523691342396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)