FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007918328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007918328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614203
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1246327.720961985574306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT659334.084554420974314Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG487643.020933550489003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGG433922.6881377373230007No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG419792.600602278647729Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT400622.4818439812093027Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC400522.4812244804401926No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG336062.0818942846717543Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC252231.5625667899266698Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT204281.2655161711383265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC183701.1380229128554462Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC161591.00105129280518Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT150690.9335257089721678Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC143090.8864436505197921Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC125500.7774734652333071Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG124110.7688624045426753Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC112330.695885213941493Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT111680.6918584589422768Clontech SMART CDS Primer II A (100% over 26bp)
CCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCAC100980.6255718766474848Clontech SMART CDS Primer II A (95% over 21bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA99890.6188193182641837Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG99880.6187573681872726Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGA99670.6174564165721412No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG93950.5820209725790375Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG77650.4810423472140741Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGTACTCTG75160.46561677806322993No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC73650.45626231644966586Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA70720.4381109439147369Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAA65110.4033569507676544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60240.3731872633119874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAA58940.36513375331355474No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG58250.36085919800669436Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA57370.35540759123852456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT55590.3443804775483629Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAG53470.3312470612432265No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA52760.3268486057825441Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC51570.31947654663013264Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC49000.3035553768640004Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC48710.3017588246335808Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTACCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTC47630.29506821632719055Clontech SMART CDS Primer II A (96% over 26bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTAACAAACCGCCCATGT42190.26136737448759545No Hit
GGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAA41550.2574025695652901No Hit
GGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGGTAAGGG40360.25003051041287866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGTTTGTTAG38590.23906534679962807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36850.22828603341711046Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36120.223763677802606Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA36100.22363977764878396Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT34620.21447116626595294Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGGGTAAGGCAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34130.2114356124973129No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC33600.20815225842102883Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTAC30260.187460932732748No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT28560.1769294196578745Clontech Universal Primer Mix Long (96% over 26bp)
CCCTAACAAACCGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT28560.1769294196578745Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT25390.15729124527708102Clontech SMART CDS Primer II A (100% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA23720.1469455824329406Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22820.14137007551094874No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22720.14075057474183855Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA22470.13920182281906304Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22270.1379628212808426Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21900.13567066843513487Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20740.1284844595134565Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20070.1243338043604181Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT19520.12092655013031198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT19120.11844854705387116Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.11689979513109566No Hit
GTATCAACGCAGAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAA18380.11386424136245565No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT17850.11058088728617156Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGCGGTTTGTTAGGGTAAGGGTAAGGGTAAGGGTAAGGGTA17260.10692583274842136No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA16710.10351857851831524Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACCCTTACC16630.10302297790302707No Hit
GGGTAAGGGTAAGGGTAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16330.10116447559569644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)