FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007919185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007919185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27023184
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTA1076530.3983727454174164TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTAT935980.34636184988415875TruSeq Adapter, Index 18 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCGCGTA286680.10608668467786771TruSeq Adapter, Index 18 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)