FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007919342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007919342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493807799
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG66123061.3390444649498134TruSeq Adapter, Index 8 (97% over 35bp)
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT24178460.48963301205374443No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA16327980.3306545589815604No Hit
CAGCGCTGGTTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6220410.12596824133998744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)