FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007919344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007919344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486591397
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG59464251.2220571585650126TruSeq Adapter, Index 8 (97% over 35bp)
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT23373740.4803566224990205No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA15861570.3259730874362335No Hit
CAGCGCTGGTTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6220650.1278413477581479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)