FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007919376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007919376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264431017
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NAGCCGCTTTGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT6378050.2411990118390688No Hit
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCTAGATCTCGTATG4148030.1568662423591556TruSeq Adapter, Index 7 (97% over 34bp)
NAGCCGCTTTGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3717990.14060339978951863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)