FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007919932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007919932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49651837
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT2221100.44733490928039576No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1878580.37835055327358785No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG1827620.3680870860830386No Hit
GGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAG1363450.27460212599989No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG1192240.24012001811735584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG947790.19088719718466812No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT880820.17739927729159347No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA828150.1667914119672954No Hit
GAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCA823950.16594552181422814No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG797050.16052779678624982No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG793870.15988733709892747No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA754320.15192187149087757No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC736890.1484114273556485No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT732760.14757963537179902No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT728590.1467397872912537No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT717550.14451630460319123No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA702660.14151742260815045No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT699700.14092127145265543No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC689060.13877834973155173No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC674470.13583988846173004No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA661000.13312699789939292No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG643110.12952390865216123No Hit
CCCCAGGCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCACCATGGT642080.12931646416224238No Hit
CGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAG613310.12352211661373173No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG609590.12277289962101504No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA606150.12208007530516947No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT590190.11886569272351394No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG586510.11812453182749312No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT585780.11797750806279332No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA585170.11785465258818117No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA578900.11659185943110222No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC572920.11538747297506838No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT570030.11480541998879115No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCC567380.11427170358268919No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT560400.1128659147092584No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG556810.1121428800308033No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT554510.11167965447079027No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC546060.10997780404378593No Hit
TGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTC540720.10890231513488616No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGAC538110.10837665482548009No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC536050.10796176584564232No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT535050.10776036342824537No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC531610.10706753911239981No Hit
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACC527270.106193452620897No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA525380.10581280205201674No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA516020.10392767542518114No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA515780.10387933884500587No Hit
AAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATA504620.10163168786685575No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGG501050.1009126812367486No Hit
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC498500.10039910507238634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)