FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007920075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007920075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55458421
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2757510.49722115240172454No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC2609820.47059039059189944No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2535940.45726869865263564No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1760020.317358476542273No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA1724460.3109464656413496No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1469320.2649408283730256No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG1376080.24812823286115554No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1375330.24799299641077052No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT1236480.2229562215628173No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA1205900.21744218069245067No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1204740.2172330149825218No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC1119900.20193506771496433No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA1117810.20155820880655798No Hit
GACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA1047730.18892171488257842No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG1009920.18210399463049987No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT986990.17796936555406076No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA955040.17220829276765742No Hit
CCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGG933670.16835495550801924No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG922890.16641115692781805No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA908820.16387412111859442No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC891090.1606771314314917No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC875730.15790748892760578No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA874350.15765865385889727No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT862960.15560486296571624No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT850580.15337255995802695No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG820220.1478981884464399No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT815100.14697497427847792No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC811680.1463582960647221No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA811120.14625731951510124No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC783140.14121209833940276No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA766640.13823689643093157No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG764330.13782036816374557No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG743240.13401751917891783No Hit
CGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG740880.13359197514837287No Hit
CCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCT739440.13333232116363355No Hit
GGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATA732860.13214584670558868No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC732150.1320178228658908No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT720450.12990813423988395No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA716190.12913999120169686No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT714170.1287757543619931No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT705930.1272899565604293No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG701040.12640821490391874No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT696630.12561302457565462No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG693660.1250774882321298No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA689110.12425705376646046No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG685010.12351776117102216No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT684720.12346546974353993No Hit
GGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTC674730.121664120224411No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG670590.1209176150182855No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG668150.12047764576636612No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA662920.11953459691901433No Hit
GAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGT660270.11905676146098715No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC659860.11898283220144332No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG657030.1184725399953237No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC655750.11824173645333322No Hit
GAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCT652140.11759079833881314No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG645890.11646382791893768No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT635910.11466428155248054No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG621060.11198659983485647No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG616230.1111156770943767No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG614690.1108379915829194No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT613320.11059095966688269No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA612520.11044670745313863No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC610790.1101347620409171No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT610350.11005542332335787No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA609410.1098859269722086No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG608290.10968397387296693No Hit
TGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA606180.10930350865921697No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG603890.10889058669737459No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA601000.1083694755752242No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC600490.10827751478896236No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT597520.10774197844543752No Hit
GACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATT593650.10704415836145065No Hit
GGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTT589870.10636256665150998No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC588120.10604701493394483No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC587650.1059622667583702No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG584010.10530591918583475No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT579740.10453597299497583No Hit
ACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCC573430.10339818365906955No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG563210.1015553616284892No Hit
AAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGAC559120.10081787218572269No Hit
GAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCG556350.1003183988956339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)