FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007923499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484906
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT39590.8164468989866077No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG21150.4361670096884757No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20200.41657558372138104No Hit
ACCTGCACCAACTACGACCGGTTCCTTCAGCGGAGTGTGCAGTT17340.3575950802836013No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG16620.3427468416559086No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16230.33470404573257495No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG14860.30645114723265954No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG13960.2878908489480435No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA13820.28500369143710325No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT13040.2689180995904361No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG12680.2614939802765897No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG11840.2441710352109481No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11760.24252123091898226No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9260.19096484679504894No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8220.16951739099949267No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG7320.1509570927148767No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7310.15075086717838096No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG7240.14930728842291083No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7100.14642013091197056No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG7070.14580145430248337No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7030.14497655215650043No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6710.13837733498863697No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG6140.12662247940838017No Hit
GGCTGGAATGGATTCGGAGTTGCGCGGGCCGGTCGGCAGGGTGT6050.12476644957991859No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG6050.12476644957991859No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5700.11754855580256793No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT5650.11651742812008926No Hit
TTCGATGGTATCCGGTTCGTCAGAGTTGCTTTCCAGTGCCAGTT5590.11528007490111485No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5500.11342404507265326No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5490.11321781953615752No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5490.11321781953615752No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5470.11280536846316605No Hit
GTGTGCAGTCAGGCCGCCGCCTTTCTGCGGGGTGTTGATCAGTT5340.11012443648872151No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG5170.10661860236829405No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5110.10538124914931968No Hit
TTCCTCACCGGCAGCTTTTGCCTGTTCTTCAATGTTAGCAACAA5020.10352521932085806No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT4990.10290654271137085No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT4870.10043183627342206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)