FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007923504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510442
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56581.1084511070797465No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC53271.0436053459550743No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG39370.7712923309602266No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT27460.5379651360977349No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23780.4658707551494587No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23740.465087120573934No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23440.4592098612574984No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23260.45568350566763705No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17540.3436237613675991No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16950.33206515137860915No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16690.32697152663769835No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16420.3216819932529063No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15900.31149474377108466No Hit
ATGTCCAACATGGACATCGATGGTATTAACA15260.2989565905626888No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG15000.293862965821778No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14200.2781902743112831No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14140.2770148224479961No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13530.26506439517124375No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13460.26369303466407545No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13380.26212576551302597No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12920.2531139678944915No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12580.24645307400253114No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11070.21687086877647216No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10440.20452862421195747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9210.18043186101457168No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9200.1802359523706905No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA9170.17964822643904693No Hit
ATGATCATCAACCACAACACCAGCGCAATCA9110.17847277457575983No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8750.17142006339603716No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8610.16867734238170057No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8340.16338780899690855No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8190.1604491793386908No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8140.15946963611928486No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7890.15457192002225523No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA7700.1508496557885127No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7650.14987011256910676No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG7160.1402705890189287No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7150.1400746803750475No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6700.13125879140039415No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6670.1306710654687506No Hit
GCTGAAACCGGCGAAACCAAAATTGTTGAGC6460.12655698394724574No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5910.11578200853378054No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5720.112059744300038No Hit
CAGGGTGCAGACGAACAGACCATCGGCTCCG5640.11049247514898852No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5590.10951293192958259No Hit
ATGGTAGTTCAGCACAATATGCAGGCTATGA5380.1053988504080777No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5270.10324385532538467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)