FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007923505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484424
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC36180.7468663815170182No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT35870.7404670288837878No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23140.47768070946113317No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23110.4770614172708206No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA22990.47458424850957015No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA21860.4512575760077948No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21360.44093603950258453No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19310.39861773983122223No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT18130.3742589136789259No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14850.30654963420474624No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG14430.2978795435403696No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC14300.29519594404901495No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14240.29395735966838965No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG14090.2908608987168266No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC13640.2815715158621373No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13600.2807457929417205No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT13010.2685663798655723No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12270.25329050583786106No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC11970.24709758393473483No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11360.23450530939837827No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10090.20828860667514407No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9400.19404488629795386No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA9340.19280630191732862No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8680.17918187373045102No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC8480.1750532591283669No Hit
GGTGGAAGCCTGATCCAGGCCTTTAATCTGTTTGCGCATCTTTT8460.17464039766815848No Hit
AGTAGAGGCACGGTCCAGACCCTTGATTTGTTTGCGCATTTTTT8070.1665895991940944No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7970.16452529189305237No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7820.16142883094148927No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7720.1593645236404472No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7430.15337803246742523No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7140.14739154129440327No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6700.13830858916981817No Hit
GCTAGAGGCCTGAGACAGGCCGCGGATCTGCTTACGCATTTTTT6530.13479926675804668No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6310.13025779069575413No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6290.12984492923554572No Hit
GTTGTCAGACGCTTTGTTCAGGCCACGAATCTGGGAGCGCATTT6180.12757419120439945No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6130.12654203755387844No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG6090.1257163146334616No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5950.1228262844120027No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5880.12138126930127326No Hit
ACGGGACGCCTGGCTCAGGCCGCGAATCTGTGCGTTAATTTTGC5820.12014268492064803No Hit
TGCACCTACTTCACGGCAGACGCGGCTTTCGGAACGGCCCGCAC5680.11725265469918913No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5600.11560120885835548No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5500.11353690155731344No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5420.11188545571647979No Hit
CGGCAGCAGCAGCTTCTGGCCGATATAAATCAGGTTGCCGTCGG5390.11126616352616717No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG5260.10858256403481246No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT5180.10693111819397882No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5010.10342179578220732No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5000.10321536505210312No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4920.10156391921126948No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4920.10156391921126948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)