FastQCFastQC Report
Wed 31 May 2023
EGAF00007923506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484424
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52081.075091242382706No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48871.0088269780192558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47660.9838488596766469No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG36910.7619358248146252No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT25520.5268112232259343No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22650.4675656036860271No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22640.46735917295592294No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22450.46343698908394304No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22430.46302412762373457No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16780.34639076511485806No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15810.3263669842947501No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15120.3121232639175598No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15010.30985252588641354No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14350.29622809769953595No Hit
ATGTCCAACATGGACATCGATGGTATTAACA14340.2960216669694317No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG14210.29333806747807706No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13610.28095222367182465No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13310.2747593017686985No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12990.26815351840536394No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12570.2594834277409872No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12380.2555612438690073No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11710.24173038495202548No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11700.2415239542219213No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9880.20395356134295575No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9560.19734777797962116No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA8780.18124618103149306No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8660.17876901227024258No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8490.1752596898584711No Hit
ATGATCATCAACCACAACACCAGCGCAATCA8130.16782818357471968No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8020.1655574455435734No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7910.16328670751242713No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7830.16163526167159348No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7660.158125939259822No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7350.15172658662659158No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA7310.15090086370617475No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6820.14078575793106865No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG6420.1325285287269004No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6390.1319092365365878No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6010.12406486879262796No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5870.12117483857116906No Hit
GCTGAAACCGGCGAAACCAAAATTGTTGAGC5830.12034911565075224No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5770.11911053127012698No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5640.11642693177877231No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5620.11601407031856391No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5460.11271117863689661No Hit
ATGGTAGTTCAGCACAATATGCAGGCTATGA5040.10404108797251994No Hit
CAGGGTGCAGACGAACAGACCATCGGCTCCG5020.10362822651231152No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5020.10362822651231152No Hit
GAAGTTCACACCATGGTTGTACAGCACAACA5010.10342179578220732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)