FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007923508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516246
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54201.04988706934291No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC53451.0353591117413015No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG40150.7777299969394436No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28750.5569050413949939No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25850.5007302720021075No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25330.49065755473165895No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24350.47167435679889047No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG24120.46721911646773046No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17590.34072903228305884No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17480.3385982651681563No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC16380.31729059401913046No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16280.3153535330055826No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG15840.30683046454597224No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15840.30683046454597224No Hit
ATGTCCAACATGGACATCGATGGTATTAACA15160.2936584496538472No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG15120.29288362524842804No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14490.2806801408630769No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG14270.2764186066332717No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13730.26595847716011356No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13680.2649899466533397No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12990.25162422565985987No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12730.24658786702463553No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11120.2154011847065159No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10400.2014543454089717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10000.19370610135478047No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA9640.18673268170600837No Hit
ATGATCATCAACCACAACACCAGCGCAATCA9130.17685367053691456No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC9090.17607884613149544No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8910.1725921363071094No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8590.16639354106375642No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8380.16232571293530604No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8270.16019494582040344No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA8050.1559334115905983No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7790.150897052955374No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7780.1507033468540192No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7750.15012222854995486No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7040.13636909535376546No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG6920.1340446221375081No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6350.1230033743602856No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6330.12261596215757603No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6320.12242225605622126No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5970.11564254250880394No Hit
GCTGAAACCGGCGAAACCAAAATTGTTGAGC5840.1131243631911918No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5620.10886282896138662No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5430.10518241303564578No Hit
CAGGGTGCAGACGAACAGACCATCGGCTCCG5350.10363276422480755No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5260.10188940931261453No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5180.10033976050177629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)