FastQCFastQC Report
Sun 21 May 2023
EGAF00007923510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492438
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT52461.065311775289478No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48870.9924091966907509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41630.845385611995825No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG35980.7306503559839005No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26500.538138811383362No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24410.4956969202214289No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA22630.4595502377964332No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21960.44594446407466526No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG21920.4451321790763507No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC17080.3468456942802952No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16640.33791055929883557No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15230.30927751310824914No Hit
ATGTCCAACATGGACATCGATGGTATTAACA15210.3088713706090919No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC15150.30765294311162017No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG14530.29506252563774527No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14530.29506252563774527No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13830.2808475381672414No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13670.2775983981739833No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13160.2672417644454733No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12600.2558697744690702No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12490.2536359907237053No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12360.25099606447918316No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11910.24185785824814496No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10210.20733574581977832No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9590.19474532834590344No Hit
ATGGTGGTCCAGCACAATCTGCAGGCCATGA9480.19251154460053854No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA8610.174844345887198No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8610.174844345887198No Hit
ATGATCATCAACCACAACACCAGCGCAATCA8230.16712763840321015No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8180.16611228215531704No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8060.1636754271603735No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7980.16205085716374448No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7590.154131078430178No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7580.1539280071805994No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7490.15210036593439175No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA7160.14539901469829702No Hit
GTCGTTCAGCACAATATGTCTGCCATGAACG6990.14194680345546037No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6440.13077788472863588No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6350.12895024348242823No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6170.12529496099001294No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5920.12021817975054727No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5820.11818746725476101No Hit
GCTGAAACCGGCGAAACCAAAATTGTTGAGC5770.11717211100686786No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5330.10823697602540827No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5270.1070185485279365No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5120.1039724797842571No Hit
ATGGTAGTTCAGCACAATATGCAGGCTATGA5080.1031601947859426No Hit
CAGGGTGCAGACGAACAGACCATCGGCTCCG5050.10255098103720672No Hit
GAAGTTCACACCATGGTTGTACAGCACAACA4990.10133255353973496No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4950.10052026854142045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)