FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007923523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436956
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG33470.7659810141066835No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32340.7401202867107901No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA29560.6764983201969993No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG27810.6364485211325626No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14400.3295526323016505No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT13110.30003020899129434No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12910.2954530890982158No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC11490.26295553785735865No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10740.24579133825831434No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG10660.24396049030108294No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7620.17438826792629006No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7410.16958229203855765No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7280.16660716410805665No Hit
TTTAGTGCCGTTGACTGCAATTTCAACACGGCGGTCCGGCGCCA7140.1634031801829017No Hit
CTGGTCCATGGTCTCTTTCGGCATTGCGCCGTAAGTGGTCAGGT7070.16180118822032424No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT6970.15951262827378498No Hit
GGAGCTCGGACCTACGTTGGTAACAGTGAACACGTCCGGACGCG6690.15310466042347512No Hit
GTCAATGCACGCGCCAGAGTTGAAGGTCTGAGTAGCAGAGCTGC6630.15173152445555158No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6350.1453235566052417No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6090.1393733007442397No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6040.13822902077097007No Hit
CATGGTTGCGGCGGTAGATGCCGCGTTCACGTCAACGCGCAGTT5640.12907478098481312No Hit
GTCGATGCAAGCACCGGAGTTGAAGGTCTGGGTGGCGCTAGAAC5500.12587079705965817No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5310.12152253316123363No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5120.11717426926280906No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4930.1128260053643845No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG4880.1116817253911149No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC4630.1059603255247668No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC4540.10390062157288149No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT4450.10184091762099616No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4400.10069663764772653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)