FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007923526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484663
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT81511.681787138692246No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC37800.7799233694340191No Hit
ATGTCCAACATGGACATCGATGGTATTAACA31430.648491838659027No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28950.5973222630982765No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15560.3210478208569666No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT15150.3125883345747457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15070.31093770310504415No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC13660.28184532345155294No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13660.28184532345155294No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12700.2620377458151334No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10560.21788335400061484No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG8880.18322009313688067No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8440.17414162005352174No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA8290.1710466860478312No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC8210.16939605457812953No Hit
ATGGTTGGTGGTGAAGCCGGCAAAGAAGCGG7950.16403150230159927No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7610.15701631855536735No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7250.14958847694171No Hit
CTGAACGTACGTCCGGGCAAAACTGTTTCCG7230.14917581907428462No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC6760.13947835918978754No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6150.12689229423331264No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC6060.12503533382989832No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6020.12421001809504748No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5700.11760749221624098No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5570.11492521607797583No Hit
CCAGGTACCATCAAAAGCCGCGACATGTCTT4920.10151383538665011No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA4880.1006885196517993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)