FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007923528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451993
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75011.6595389751611198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58921.3035600108851244No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC34570.7648348536371139No Hit
ATGTCCAACATGGACATCGATGGTATTAACA28250.6250096793534413No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26160.5787700251995053No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13990.30951806775768653No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC13170.29137619387910874No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT13060.2889425278710069No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12380.2738980470936497No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12310.2723493505430394No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9600.21239266979798357No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8090.17898507277767575No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG8020.17743637622706548No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC7100.15708207870475868No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA7070.15641835161164No Hit
ATGGTTGGTGGTGAAGCCGGCAAAGAAGCGG7070.15641835161164No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC6990.1546484126966568No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC6380.14115262846990995No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6330.14004641664804543No Hit
CTGAACGTACGTCCGGGCAAAACTGTTTCCG5900.13053299498001075No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5840.12920554079377336No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5670.12544442059943406No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5220.11548851420265357No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4930.10907248563583949No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4910.10863000090709371No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC4750.10509012307712731No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC4580.101329002882788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)