FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007923704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007923704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462576
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT124692.695557054408357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66021.427224931686901No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15880.3432949396423507No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15670.33875514510048077No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG12690.2743332987444226No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10670.23066479886548374No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10460.22612500432361385No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9230.19953477914980455No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8530.18440213067690497No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7150.1545691951160458No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6840.147867593649476No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6770.14635432880218602No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6680.14440870256995608No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6240.13489675210127633No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5900.12754660855729652No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5900.12754660855729652No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5860.12668188578741654No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5770.1247362595551866No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5750.12430389817024662No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5580.12062882639825671No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5560.12019646501331674No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5310.11479194770156688No Hit
ATGGAACGTCAGCTGCCAAAAAACGTTCGCC5140.11111687592957697No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5130.11090069523710698No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5110.11046833385216699No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5090.110035972467227No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5050.10917124969734703No Hit
ACTCGTCTGGAAAACATCAAAACCGACCGTA4940.1067932620801771No Hit
GCATCTTATGTAACCGACGCTAGCAAATGCA4900.10592853931029712No Hit
CCGAAAGACCAGGAACGTAAACGTCGTTTCA4890.10571235861782712No Hit
CTGGAACGTGTTACCAAACAGTCTTACGGTA4710.10182110615336723No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA4700.10160492546089724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)